Louis et al 2008

 

Louis EE, Engberg SE, McGuire SM, McCormick MJ, Randriamampionona R, Ranaivoarisoa JF, Bailey CA, Mittermeier RA, Lei R. (2008). Revision of the Mouse Lemurs, Microcebus(Primates, Lemuriformes), of Northern and Northwestern Madagascar with Descriptions of Two New Species at Montagne d’Ambre National Park and Antafondro Classified Forest. Primate Conservation. 23: 19-38.

 

Appendix I

• (a) Microcebus table of individual samples and corresponding information for each sample (bar code number, site, original species designation, current species designation, GenBank accession numbers of sequence data).
• (b) Field notes for Microcebus margotmarshae (formerly Microcebus sp. nova #5) and Microcebus arnholdi (formerly Microcebus sp. nova #6).

 

Appendix II

• (a) Maximum parsimony phylogram derived from the D-loop sequence data from 82Microcebus individuals with 18 out-group taxa (one of 3886 most parsimonious trees). Values above branches indicate number of changes between nodes. Values within the circles along the branches indicate support of bootstrap pseudoreplicates. Length=2052; CI = 0.4235; RI = 0.8460; RC = 0.3583; HI = 0.5765.
• (b) Part A. Neighbor-joining phylogram derived the D-loop DNA sequence data from the 121 Microcebus individuals with18 out-group taxa. Values above branches indicate number of changes between nodes. Values within circles indicate support of bootstrap pseudoreplicates. Solid black circle indicates the branch that connects in-group taxa to the out-group taxa (displayed on next page (Part B)).
• (b) Part B. Neighbor-joining phylogram derived the D-loop DNA sequence data from the 121 Microcebus individuals with18 out-group taxa. Values above branches indicate number of changes between nodes. Values within circles indicate support of bootstrap pseudoreplicates. Solid black circle indicates the branch that connects to the in-group taxa (displayed on previous page (Part A)).
• (c) Maximum parsimony phylogram derived from the D-loop sequence data from 77Microcebus haplotypes with 18 out-group taxa (one of 364 most parsimonious trees). Values above branches indicate number of changes between nodes. Values within the circles along the branches indicate support of bootstrap pseudoreplicates. Length=2138; CI = 0.4574; RI = 0.8578; RC = 0.3924; HI = 0.5426.
• (d) Fifty percent majority-rule consensus phylogenetic tree from the Bayesian analysis derived from the D-loop sequence data from 77 Microcebus individuals with 18 out-group taxa reconstructed using the program MrBayes. Branches without posterior probability values (PP) are supported by less than 50% of the sampled trees.
• (e) Maximum parsimony phylogram derived from the D-loop and PAST sequence data from 89 Microcebus individuals with 18 out-group taxa (one of 4112 most parsimonious trees). Values above branches indicate number of changes between nodes. Values within the circles along the branches indicate support of bootstrap pseudoreplicates. Length=6539; CI = 0.4271; RI = 0.8755; RC = 0.3739; HI = 0.5729.
• (f) Microcebus margotmarshae, Margot Marsh’s or Antafondro mouse lemur at Antafondro Classified Forest (Maromiandra). Photo by Rambinintsoa Andriantompohavana.
• (g) Microcebus arnholdi, Arnhold’s or Montagne d’Ambre mouse lemur at Montagne d’Ambre National Park and Classified Forest. Photo by Edward E. Louis Jr.
• (h) Distribution map of the mouse lemurs of Madagascar. Designated sites and species are based on molecular genetic data. The species legend corresponds to the color coded to the sites.

Appendix III

• (a) Summary of the acronyms and GenBank accessioned sequences used in this study.
• (b) Table 1A. Diagnostic nucleotide sites from the D-loop Pairwise Aggregate Analysis (PAA) of genus Microcebus.
• (c) Table 1B. Diagnostic nucleotide sites from the PAST Pairwise Aggregate Analysis (PAA) of genus Microcebus.
• (d) Haplotypes for Microcebus D-loop Sequences.
• (e) Haplotypes for Microcebus PAST Sequences.